Table 1. General statistics for RNA concentration (in micrograms) and RNA integrity number (RIN) data per mL of blood.
The sample collection tube followed by RNA extraction procedure is listed in the rows. The PBMC separation procedure is also referred as PBMC prep. CV refers to coefficient of variation and SD refers to standard deviation and μg refers to microgram.
| Sample | Whole Blood | PBMC | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tube | EDTA | ACD-A | RNAgard® | PAXgene® | EDTA | CPT | ACD-A | |||
| RNA Extraction | TRIzol® LS |
ACK_miRNA | TRIzol® LS |
ACKmi RNA | Biomaxi™_PAXmiRNA | PAXmiRNA | TRIzol | |||
| PBMC Prep | LeukoLOCK® | LSM | CPT | Magnetic | ||||||
|
Mean yield (μg) |
18.4 | 0.9 | 31.1 | 0.2 | 3.7 | 1.7 | 1.1 | 5.9 | 2.4 | 2.3 |
| Standard deviation | 10.2 | 0.9 | 21.0 | 0.2 | 1.5 | 0.4 | 0.8 | 2.2 | 1.6 | 0.8 |
| Range | 24.7 | 2.0 | 54.1 | 0.4 | 4.0 | 1.0 | 2.0 | 5.3 | 4.2 | 2.0 |
| CV | 55.4 | 104.1 | 67.5 | 74.9 | 38.9 | 26.0 | 73.0 | 36.6 | 65.8 | 35.0 |
| Mean RIN | 5.6 | 7.6 | 5.1 | 7.8 | 4.9 | 8.2 | 8.2 | 8.8 | 8.4 | 8.8 |
| RIN SD | 1.3 | 1.7 | 1.4 | 1.3 | 0.9 | 0.4 | 0.4 | 0.3 | 1.4 | 0.3 |