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. Author manuscript; available in PMC: 2020 Aug 15.
Published in final edited form as: Life Sci. 2019 Jun 14;231:116571. doi: 10.1016/j.lfs.2019.116571

Fig. 2.

Fig. 2.

CDKN2B-AS1 forms circular splice forms that are altered in ulcerative colitis patients with active inflammation. (a) A graphical representation of both linear and circular CDKN2B-AS1 and primer sets used to detect them. (b) RNA was purified from the colonic epithelial cell line, Caco2 BBE and was subjected to either RNase R or RNase R buffer treatment. Subsequent RNA was reverse transcribed and qPCR was performed against the linear form of CDKN2B-AS1 or the circular form, exons 7–5. Mean ± SEM. 3 independent experiments. *** p < 0.001. (c) Whole colon biopsy RNA from healthy controls or inflamed Ulcerative colitis patients was reverse transcribed and outward facing primers against exons 7 and 5 were amplified. Mean ± SEM. n = 17 controls and 19 UC samples. * p < 0.05.