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. Author manuscript; available in PMC: 2020 Aug 15.
Published in final edited form as: Life Sci. 2019 Jun 14;231:116571. doi: 10.1016/j.lfs.2019.116571

Fig. 5.

Fig. 5.

Down-regulation of CDKN2B-AS1 in colonic epithelial cells does not affect p15 levels or PES1 localization. (a) RNA was harvested from control shRNA (shCtrl) Caco2 BBE cells and Caco2 BBE cells containing two different shRNA sequences (shT1, shT2) against CDKN2B-AS1. qPCR was performed against p15 and HPRT1 to normalize samples. Mean ± SEM. 3 independent experiments. (b) After harvesting protein from sub-confluent cells western blots were performed against p15 and actin. (c) Pes1 immunostaining in shCtrl, shT1 and shT2 cells. Scale Bar = 50 μm. Double blinded images were quantified by three separate reviewers and the experiment was replicated 3 times. Mean ± SEM.