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. Author manuscript; available in PMC: 2019 Dec 7.
Published in final edited form as: Biochemistry. 1991 Sep 17;30(37):8945–8953. doi: 10.1021/bi00101a005

Table II:

Multiexponential Analysis of Intensity Decays for Nuclease-ConA-S28Ga

temp (°C) τ1 (ns) α1 τ2 (ns) α2 τ3 (ns) α3 χR2
10 5.34 1.0 82.8
6.11 0.792 1.38 0.208 2.2
6.61 0.597 3.80 0.261 0.85 0.143 1.6
20 4.92 1.0 89.4
5.69 0.777 1.31 0.223 2.1
6.27 0.520 3.79 0.313 0.84 0.157 1.5
25 4.54 1.0 142.7
5.35 0.749 1.00 0.251 2.1
6.06 0.461 3.77 0.334 0.73 0.205 1.5
30 3.89 1.0 311.1
4.82 0.669 1.75 0.331 2.6
5.31 0.482 2.92 0.233 0.55 0.285 1.3
35 2.67 1.0 599.2
3.71 0.550 0.53 0.450 2.2
4.35 0.284 2.76 0.294 0.46 0.422 1.1
40 1.84 1.0 583.1
2.74 0.485 0.44 0.515 3.3
3.59 0.162 2.07 0.362 0.34 0.476 1.4
45 1.45 1.0 558.0
2.12 0.475 0.31 0.525 5.1
3.27 0.090 1.69 0.417 0.24 0.493 1.1
Global Analysis
Ea,1 (kcal/mol) A1 (ns−1) Ea,2 (kcal/mol) A2 (ns−1) Ea,3 (kcal/mol) A3 (ns−1) χR2
〈1.0〉 0.977 1.88 9.66 〈4.0〉 1901 4.2
a

All χR2 are calculated with δϕ = 2.0° and δm = 0.004. For the global analysis, Ea,1 and Ea,3 were fixed. The α1 and α3 for the global analysis are given in Figure 10 as the open symbols.