Table III.

Intensity Decay Analysis Before and After (Data in Parentheses) Photobleaching of mag-quin-2 and MgG

Condition	τi (ns)	αi	$f_i^a$	$\overline{\text{τ}}(\text{ns})$a	$I/I_0$b
mag-quin-2
No Mg2+	0.18	0.953 (0.932)	0.612 (0.508)
	1.23	0.039 (0.058)	0.168 (0.205)
	7.66	0.008 (0.013)	0.220 (0.287)	2.00 (2.54)	0.96
35 mM Mg2+	0.39	−0.023 (0.189)	−0.001 (0.012)
	2.54	−0.012 (0.148)	−0.007 (0.061)
	8.56	0.956 (0.663)	1.008 (0.927)	8.56 (8.09)	0.35
Magnesium green
No Mg2+	0.07	0.761 (0.780)	0.228 (0.185)
	0.46	0.216 (0.172)	0.444 (0.280)
35 mM Mg2+	3.11	0.023 (0.048)	0.328 (0.534)	1.22 (1.76)	0.95
	0.16	0.293 (0.598)	0.018 (0.063)
	3.56	0.707 (0.402)	0.982 (0.937)	3.68 (3.16)	0.60

^aFractional intensity $f_i={\alpha }_i{\text{τ}}_i/\text{Σ}{\alpha }_i{\text{τ}}_i$ and mean lifetime $\overline{\text{τ}}=f_i{\text{τ}}_i/\text{Σ}{f}_i{\text{τ}}_i.$.

^bRelative intensity of the photobleached (I) sample relative to the nonphotobleached sample (I₀).