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. 2019 Dec 6;9:18545. doi: 10.1038/s41598-019-53867-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Age and relaxin’s effect on the levels of Cx-43 and β-catenin in aged atria. (Aa–c) Aged right atrial tissue (Aa–c) expressed significantly less β-catenin and Cx43 than RLX-treated animals (Ad–f). (Ag–h) Quantification of atrial β-catenin and Cx43 expression (n = 5/group). 60x magnification. (B) RLX (Bb) increased Wnt1 expression in LV compared to control (Ba). (Bc) Quantification of Wnt1 expression in LV sections. 600x magnification, scale bars = 25 µm and apply to all panels.