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. 2019 Dec 6;9:18545. doi: 10.1038/s41598-019-53867-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relaxin inhibits DKK1 expression in Aged LV. Relaxin significantly reduced DKK1 expression compared to control hearts. C = control. R = Relaxin. n = 3/group. Gels were probed for DKK1 expression (top), stripped and re-probed for, and normalized to, α-tubulin expression (bottom). Line delineates separate probe experiments, measured on the same membrane. For full blots, see Supplementary Fig. S2.