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. 2019 Dec 6;9:18561. doi: 10.1038/s41598-019-54793-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The different substrates support keratinocyte adhesion and proliferation to varying degrees. (A) The ability of Fib-Mat, Collagen I and TCP to support keratinocyte adhesion. Shown are data from three separate experiments, where each data point is the percent of bound keratinocytes per well; means are shown as a line. *p < 0.01 (B) Morphology of keratinocytes growing on dermal fibroblast-derived matrix (Fib-Mat), collagen I (Col I) and tissue culture plastic (TCP) as captured by phase contrast microscopy. Keratinocyte on days 1, 4 and 6 post seeding are shown. Scale bars are 100 μm. (C) The ability of Fib-Mat, Col I and TCP to support keratinocyte proliferation. Nuclei were stained with DAPI and counted. The data are from 4 replicate wells of three separate experiments. Means are shown. (D) Ki67 expression by keratinocytes cultured on Fib-Mat, Col I and TCP on day 3. The data are the percent of Ki67 positive keratinocytes for each of 4 replicate wells; mean values are shown as a line. These data are representative of three separate experiments. *p < 0.01.