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. 2019 Dec 6;2:459. doi: 10.1038/s42003-019-0709-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Histogram of number of photons per burst for red channel (Atto 647N). b Fractions of coincident bursts as a function of normalized brightness threshold n_br. Obtained saturated coincidence fractions are 0.94 and 0.92 for red and blue channel, respectively. Burst and analysis statistics are given in Supplementary Notes 1 and 2. c Concept of global coincidence analysis. Number of selected red and blue bursts (solid lines) and those which show coincidence with the complementary color (dashed lines) are shown as a function of brightness threshold n_br. The optimal brightness threshold n_br,opt is depicted for each color separately (see Supplementary Note 5). The brightness threshold of the color with the higher number of coincident bursts is increased (here red) until the number of coincident bursts are equal, i.e. N_RB = N_BR as indicated by the gray dashed line. The number of red-only and blue-only bursts N_R0 and N_B0, respectively, are indicated by arrows. d Differentiation between color-specific coincidence fractions and global coincidence.