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. 2019 Dec 6;9:18546. doi: 10.1038/s41598-019-54799-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of HFD consumption on metabolic parameters of B6CF1 male mice. (a) Fasting glucose levels were determined at week 12 and 18 (n_NFD = 9, n_HFD = 9). (b) Glucose tolerance test (GTT) was performed in week 18 after i.p glucose administration (2 g/kg body weight) after 6 h of fasting (n_NFD = 5, n_HFD = 6). (c) Serum cholesterol and (d) triglycerides levels were determined after euthanasia (n_NFD = 9, n_HFD = 9). (e) Mean weight of livers from NFD- and HFD-fed male mice (n_NFD = 4, n_HFD = 3). (f) Representative micrographs of Hematoxylin and Eosin stained liver sections from NFD- and HFD-fed male mice. Bar = 50 µm. Data are expressed as median with interquartile range for (a,b), and mean ± SEM for (c–e). *p < 0.05, **p < 0.01, ***p < 0.001.