Figure 2.

DSB number is reduced in the retinas of E13.5 rag2^−/− mice. (a,b) Presence of DSBs in WT and rag2^−/− mice as determined by γH2AX immunostaining (green). Nuclei were counterstained with DAPI (cyan). Quantification of nuclear γH2AX positive foci per cell (c) and percentage of γH2AX⁺ cells (d) in dissociated retinal cells from WT and rag2^−/− mice (n > 100). (e) Density of γH2AX⁺ cells in whole-mount retinas. (f) Immunostaining for γH2AX, PCNA, and TUJ-1 in dissociated retinal cells from WT and rag2^−/− animals. The percentage of γH2AX⁺ proliferative cells (PCNA⁺) and γH2AX⁺ neurons (TUJ-1⁺) are shown. Histograms depict the mean ± SEM values. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. corresponding controls. Individual values are depicted as circles (WT) and squares (rag2^−/−). Scale bar, 5 µm in a and b.