Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 6;10:5579. doi: 10.1038/s41467-019-13346-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Crown 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — High conservation of the NP_145-156 peptide across seasonal IAV viruses. a Frequency of amino acid variation at position 146 of the NP_145–156 peptide. Pie charts represent frequency of alanine (A; white), threonine (T; gray) and valine (B, black) at position 146. Total number of analyzed sequences are indicated in the pie charts: A/H1N1 (1918–1957 n = 77, 1977–2008 n = 1132, 2009–2018 n = 9291), A/H2N2 (1957–1968 n = 119), and A/H3N2 (1986–2018 n = 13,497), avian A/H5N1 (1997–2014 n = 194) and A/H7N9 (2013–2017 n = 98). b Representative FACS panels of A68/NP₁₄₅-specific CD8⁺ T cells expanded by 146A peptide stimulation on day 0, followed by restimulation at day 10 with cognate or variant NP₁₄₅ peptides. Day 10 restimulation with PMA/Ionomycin was included as a positive control and DMSO as a negative control. c Percentage of maximum IFNγ⁺CD3⁺CD4⁻ T cells following secondary stimulation. Maximum IFNγ is defined as IFNγ⁺CD3⁺CD4⁻ T cells following cognate restimulation with peptide pool (pool), DATYQRTRALVR (146A), DTTYQRTRALVR (146T) or DVTYQRTRALVR (146V) peptides, respectively. Pool included 146A, 146T, and 146V variants. The bar indicates the mean response of three donors (n = 3).