Table 2.
Frequencies of paired TRBV-TRBJ/TRAV-TRAJ expanded clonotypes within A68/NP145 TCRs in human PBMCs.
| Clone ID | TRBV | TRBJ | CDR3b | CDR3β length | TRAV | TRAJ | CDR3a | CDR3α length | Low | Medium | High | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1b | D3b | D3a | D2b | D5a | D7 | D6 | D16a | D13a | |||||||||
| AF | TRBV20-1 | TRBJ1-1 | CSAETGNTEAFF | 7 | – | – | – | – | 10 | ||||||||
| AY | TRBV20-1 | TRBJ2-1 | CSADNVAGGPGSEQFF | 11 | TRAV13-1 | TRAJ43 | CATYDMRF | 3 | 15 | ||||||||
| AZ | TRBV6-1 | TRBJ2-1 | CASSEPRDEQFF | 7 | TRAV1-2 | TRAJ9 | CAVETGGFKTIF | 7 | 15 | ||||||||
| BA | TRBV20-1 | TRBJ2-7 | CSASQDPYEQYF | 7 | TRAV35 | TRAJ37 | CAGQTTSNTGKLIF | 9 | 10 | ||||||||
| BB | TRBV20-1 | TRBJ2-1 | CSALYPLAGPGNEQFF | 11 | TRAV26-2 | TRAJ30 | CILNRDDKIIF | 6 | 10 | ||||||||
| BM | TRBV6-6 | TRBJ2-1 | CASSSPSGVYNEQFF | 10 | TRAV4 | TRAJ4 | CLVGDLINSGGYNKLIF | 12 | 56 | ||||||||
| BN | TRBV6-6 | TRBJ2-1 | CASSSPSGVYNEQFF | 10 | – | – | – | – | 28 | ||||||||
| BT | TRBV30 | TRBJ2-7 | CAWSPAGLAMYEQYF | 10 | – | – | – | – | 17 | ||||||||
| BU | TRBV20-1 | TRBJ1-1 | CSAESGNTEAFF | 7 | – | – | – | – | 14 | ||||||||
| BV | TRBV30 | TRBJ2-7 | CAWSPAGLAMYEQYF | 10 | TRAV12-1 | TRAJ29 | CVVNANSGNTPLVF | 9 | 10 | ||||||||
| BW | TRBV6-1 | TRBJ2-7 | CASSEAGGPGYEQYF | 10 | – | – | – | – | 7 | ||||||||
| BX | TRBV29-1 | TRBJ1-4 | CSVRDISTNEKLFF | 9 | – | – | – | – | 7 | ||||||||
| BY | TRBV20-1 | TRBJ1-6 | CSAEDGNSPLHF | 7 | TRAV12-2 | TRAJ47 | CAVKYGNKLVF | 6 | 7 | ||||||||
| CK | TRBV7-8 | TRBJ2-2 | CASSDSAGELFF | 7 | TRAV6 | TRAJ4 | CALSGYNKLIF | 6 | 11 | ||||||||
| CL | TRBV7-2 | TRBJ2-5 | CASSSIGVAGEETQYF | 11 | TRAV12-1 | TRAJ11 | CVVNVLLNSGYSTLTF | 11 | 11 | ||||||||
| CM | TRBV2 | TRBJ2-1 | CASNDPPGATNNEQFF | 11 | TRAV4 | TRAJ42 | CLVGGGSQGNLIF | 8 | 9 | ||||||||
| CN | TRBV28 | TRBJ1-1 | CASSSLIQANTEAFF | 10 | TRAV12-1 | TRAJ42 | CVVNVGYGGSQGNLIF | 11 | 9 | ||||||||
| CO | TRBV7-2 | TRBJ2-7 | CASSDLAGTSGTNTYEQYF | 14 | TRAV4 | TRAJ21 | CLVGGNFNKFYF | 7 | 6 | ||||||||
| CP | – | – | – | – | TRAV12-1 | TRAJ11 | CVVNRVENSGYSTLTF | 11 | 6 | ||||||||
| CQ | TRBV7-8 | TRBJ2-2 | CASSDSAGELFF | 7 | TRAV8-1 | TRAJ6 | CAANSGGSYIPTF | 8 | 4 | ||||||||
| CR | TRBV4-2 | TRBJ2-5 | CASSQVGTTLETQYF | 10 | TRAV29 | TRAJ29 | CAASAISGNTPLVF | 9 | 4 | ||||||||
| CS | TRBV9 | TRBJ2-6 | CASSVNPAQGSGANVLTF | 13 | TRAV9-2 | TRAJ34 | CALIYNTDKLIF | 7 | 4 | ||||||||
| DK | TRBV9 | TRBJ2-1 | CASSVDLKAGEEGEQFF | 12 | TRAV19 | TRAJ9 | CALSNTGGFKTIF | 8 | 95 | ||||||||
| % expanded αβ pairs of total TCRαβ repertoire | 0 | 0 | 0 | 10 | 50 | 84 | 62 | 64 | 95 | ||||||||
| Total number αβ pairs | 5 | 4 | 22 | 20 | 20 | 32 | 29 | 47 | 19 | ||||||||
CDR3 length was calculated based on underlined CDR3 sequenceD donor
aIndicates that TCR clonotypes were established on T cell lines