Fig. 3. Impact of s²C methylation on translation.

a Long-term effects of 20 mM MMS stress on sfGFP synthesis in E. coli WT cells. Single cell fluorescence was determined for a minimum of 300 E. coli cells producing sfGFP under control of the L-arabinose inducible promoter (pBAD24). Fluorescence was measured 1 and 5 h after addition of arabinose to unstressed cells or cells pre-exposed to 20 mM MMS stress for 1 h. (box plots represent the 5-95% percentile) b Illustration of the used sfGFP constructs. The serine on amino acid position 2 of the used sfGFP variant was encoded by different codons: AGC, AGT and TCC. c Influence of codons on sfGFP synthesis after exposure of cells to MMS stress. Fluorescence was measured after 1 h of induction with arabinose. The fold change was determined by dividing the mean fluorescence of stressed cells (20 mM MMS) by the mean fluorescence of unstressed cells. All experiments are from n = 3 biol. replicates and error bars reflect standard deviation. Source data are provided as a Source Data file.