Fig. 5. Glyoxalase I supports NSCLC proliferation under hypoxic conditions and in vivo.

a Western blot analysis of proteins containing the MG-H1 epitope were performed on lysates collected from 3553T3 parental cells and Glo1-deleted clones that had been cultured under normoxic and hypoxic (0.5% O₂) conditions for 72 h. b Proliferation rate of 3553T3 parental cells and Glo1-deleted clones when cultured under normoxic and hypoxic (0.5% O₂) conditions. Values indicate mean ± SEM, and P values were calculated by unpaired, two-tailed Student’s t-test (n = 3). c Tumor growth over time of allografts generated from 3553T3 parental cells and Glo1-deleted clones. The tumor volume for each genotype is depicted as a box and whisker plot for each time point. The difference in tumor growth between parental cell lines and Glo1-deleted clones was significant for every comparison (P < 0.0001 by two-way ANOVA; n = 6). d Western blot analysis using an antibody raised against the MG-H1 epitope of lysates from tumor allografts harvested at the end point of the experiment shown in d.