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. 2019 Dec 6;9:18544. doi: 10.1038/s41598-019-54361-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Abnormal morphology of starch granules in the endosperm of the Osatg7-1 mutant. (a–f) The ultrastructure of mature seeds in WT (left) and the Osatg7-1 mutant (right). Close up of central (b,e) and lateral (c,f) regions of whole images (a,d). Images were taken by SEM. Scale bars: 500 μm (a,d) and 30 μm (b,c,e,f). Starch granules at the lateral region of the endosperm were smaller and sparser in the Osatg7-1 mutant than in WT. (g–h) Close up of starch granules at the lateral region. Images were taken by EPMA. Scale bars: 2 μm. The starch granules of the Osatg7-1 mutant had small pits. Similar results were obtained from 5 independent samples.