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. 2019 Dec 6;9:18540. doi: 10.1038/s41598-019-55043-8

Figure 7.

Figure 7

Effect of eugenol on MAP Kinases activation in PMA-stimulated neutrophils. Cells were incubated for 30 min at 37 °C either without or with increased concentrations of EUG (0, 2.5, 5, 10, 20 µg/mL), followed by PMA-stimulation for 8 min. Cell lysates were analyzed by SDS-PAGE and western blot using anti-phospho-ERK 1/2, anti-phospho-p38 and anti-β-actine antibodies. (A) Western blots from different experiments were scanned and the intensity of bands was expressed relative to β-actine amount. The cumulated data of phospho-p38 (B) and phospho-ERK1/2 (C) is shown in the histogram as percentage to control (PMA alone 100%). Data are expressed as mean ± SEM of three or more separate experiments. p < 0.005 as compared to control (EUG untreated cells; 100%).