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. 2019 Dec 6;9:18540. doi: 10.1038/s41598-019-55043-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of eugenol on MEK1/2 and Raf phosphorylation in fMLF-stimulated neutrophils. Cells were incubated for 30 min at 37 °C either without or with increased concentrations of EUG (0, 2.5, 5, 10, 20 µg/mL), followed by fMLF-stimulation for 15 s. Cell lysates were analyzed by SDS-PAGE and western blot using anti-phospho-MEK 1/2, anti-phospho-Raf and anti-β-actine antibodies. (A) Western blots from different experiments were scanned and the intensity of bands was expressed relative to β-actine amount. The cumulated data of phospho-MEK 1/2 (B) and phospho-Raf (C) is shown in the histogram as percentage to control (FMLF alone 100%). Data are expressed as mean ± SEM of three or more separate experiments. p < 0.005 as compared to control (EUG untreated cells; 100%).