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. 2019 Dec 6;9:18534. doi: 10.1038/s41598-019-54582-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GeNorm stability analysis of Bsal candidate reference genes. The stability of the genes was assessed during different growth stages with (a,e,i) a combination of all growth stages (D0 + D3 + D5) (n = 18), (b,f,j) fresh spores at day 0 (D0) (n = 6), (c,g,k) sporangia at day 3 (D3) (n = 6) and (d,h,l) mature culture at day 5 (D5) (n = 6). Genes were ranked based on (a–d) the GeNorm M and (e–h) coefficient of variation (CV) value. (i–l) By pairwise variation (V) analysis, the optimal number of reference genes was determined.