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. 2019 Dec 6;9:18534. doi: 10.1038/s41598-019-54582-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo expression profile and stability of Bsal candidate reference genes. Shown is (a) the variation in the mRNA expression (Cq) of GAPDH, TUB, R6046 and α-centractin in skin (chin, belly and tail) tissue of experimentally Bsal-infected fire salamanders (n = 3). The whiskers represent the median, the minimum and maximum values, and the first and third quartiles. A plus (+) indicates the mean Cq value. (b) At euthanasia, different fungal loads were detected in the skin tissues. (c) GeNorm stability analysis providing a GeNorm M and coefficient of variation (CV) value. (d) BS_08640 and BS_08614 mean fold change in mRNA expression profiles. The data shows the normalized target gene quantities in skin tissue from Bsal-infected Salamandra salamandra, relative to freshly collected spores which is considered 1. The results are presented as means + SEM with significant differences shown by the P value.