Table 1.
Comprehensive ranking of reference gene stability.
| D0 + D3 + D5 | rank | GeNorm | RefFinder | D0 | rank | GeNorm | RefFinder |
|---|---|---|---|---|---|---|---|
| M value | Geomean | M value | Geomean | ||||
| Bd | 1 | APRT (0.58) | R6046 (1.86) | Bd | 1 | Ctsyn1 (0.16) | Ctsyn1 (1.50) |
| 2 | TUB (0.58) | APRT (2.63) | 2 | α-centractin (0.18) | α-centractin (2.00) | ||
| 3 | R6046 (0.65) | TUB (2.66) | 3 | GAPDH (0.19) | APRT (3.36) | ||
| 4 | GAPDH (0.73) | GAPDH (3.13) | 4 | APRT (0.29) | R6046 (3.41) | ||
| 5 | Ctsyn1 (0.78) | Ctsyn1 (4.21) | 5 | R6046 (0.32) | TUB (3.83) | ||
| 6 | α-centractin (0.83) | TEF1F (4.30) | 6 | TUB (0.37) | GAPDH (4.61) | ||
| 7 | TEF1F (0.90) | α-centractin (5.73) | 7 | TEF1F (0.46) | TEF1F (7.00) | ||
| Bsal | 1 | APRT (0.55) | α-centractin (1.57) | Bsal | 1 | GAPDH (0.27) | α-centractin (1.86) |
| 2 | Ctsyn1 (0.61) | APRT (2.21) | 2 | α-centractin (0.28) | R6046 (1.86) | ||
| 3 | α-centractin (0.68) | TUB (2.63) | 3 | R6046 (0.28) | GAPDH (2.21) | ||
| 4 | TUB (0.87) | Ctsyn1 (3.16) | 4 | APRT (0.33) | TUB (3.83) | ||
| 5 | GAPDH (1.39) | GAPDH (3.50) | 5 | TEF1F (0.39) | APRT (3.94) | ||
| 6 | R6046 (1.64) | R6046 (6.00) | 6 | TUB (0.46) | TEF1F (5.23) | ||
| 7 | TEF1F (1.82) | TEF1F (7.00) | 7 | Ctsyn1 (0.56) | Ctsyn1 (7.00) | ||
| D3 | rank | GeNorm | RefFinder | D5 | rank | GeNorm | RefFinder |
| M value | Geomean | M value | Geomean | ||||
| Bd | 1 | α-centractin (0.36) | Ctsyn1 (1.32) | Bd | 1 | APRT (0.30) | TUB (2.24) |
| 2 | Ctsyn1 (0.37) | α-centractin (2.11) | 2 | TUB (0.33) | R6046 (2.38) | ||
| 3 | R6046 (0.42) | TEF1F (3.46) | 3 | TEF1F (0.34) | APRT (2.38) | ||
| 4 | TEF1F (0.44) | TUB (3.66) | 4 | R6046 (0.39) | TEF1F (2.71) | ||
| 5 | APRT (0.49) | R6046 (4.12) | 5 | GAPDH (0.49) | GAPDH (2.94) | ||
| 6 | TUB (0.52) | APRT (4.16) | 6 | Ctsyn1 (0.66) | Ctsyn1 (6.00) | ||
| 7 | GAPDH (0.57) | GAPDH (7.00) | 7 | α-centractin (0.89) | α-centractin (7.00) | ||
| Bsal | 1 | Ctsyn1 (0.22) | TEF1F (2.00) | Bsal | 1 | TUB (0.27) | TUB (1.19) |
| 2 | APRT (0.23) | APRT (2.11) | 2 | α-centractin (0.30) | α-centractin (1.57) | ||
| 3 | α-centractin (0.24) | Ctsyn1 (2.51) | 3 | APRT (0.32) | APRT (2.71) | ||
| 4 | TEF1F (0.43) | α-centractin (2.71) | 4 | GAPDH (0.54) | R6046 (4.47) | ||
| 5 | TUB (0.56) | TUB (3.50) | 5 | R6046 (0.61) | GAPDH (5.14) | ||
| 6 | GAPDH (0.75) | GAPDH (6.00) | 6 | TEF1F (0.64) | TEF1F (6.00) | ||
| 7 | R6046 (0.95) | R6046 (7.00) | 7 | Ctsyn1 (0.71) | Ctsyn1 (6.09) |
GeNorm and RefFinder were used to determine reference gene stability in Bd and Bsal spores day 0 (D0) (n = 6), sporangia day 3 (D3) (n = 6), mature sporangia day 5 (D5) (n = 6) and a combination of all life stages (D0 + D3 + D5) (n = 18). Candidate genes were ranked from most stable (1) to least stable (7).