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. 2019 Dec 6;9:18512. doi: 10.1038/s41598-019-54366-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcription landscape throughout the T. brucei cell cycle. (A) Density plot showing the distribution of the population according to DNA content and nascent RNA synthesis. Left – Non-treated (control) population showing transcriptional activity (nascent RNA) = 38.9 ± 3.9% (red square). Right – After transcription inhibition using α-amanitin, nascent RNA decreased to 7.4 ± 0.8% (red square). The cell cycle phases (G0/G1, S, and G2/M) are indicated. Red square – transcription-positive, blue square – transcription-negative. (B) Histograms show virtually unchanged DNA content profiles for the control (black line) and α-amanitin treated groups (green line). These assays were carried out in biological triplicate, and 20,000 parasites (n = 20,000) were counted in each analysis.