Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 6;9:18512. doi: 10.1038/s41598-019-54366-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Model showing consequences of the transcription activity during the S phase in T. brucei. During the S phase, transcription can generate replication-transcription conflicts and R-loops. Our findings did not allow us to distinguish if R-loops are a consequence of replication-transcription conflicts or the cause of replication hindering, However, according to our data, both conflicts and R-loops contribute to the presence of endogenous DNA lesions (γH2A) and backup-origins firing, helping to maintain robustness in S-phase duration. The backup-origins firing may help to explain the discrepancy observed by us regarding the number of constitutive origins are not enough to allow a complete DNA replication within the S-phase duration (Fig. 2). Further studies are necessary to investigate if the activation of the backup origins occurs in an active (triggered by replication stress) or passive (stochastic) manner.