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. 2019 Dec 6;10:5598. doi: 10.1038/s41467-019-13609-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Liquid-phase separation of Smp proteins and its impact on homologous pairing. a–c Selected projected time-lapse images of live cells at the horsetail stage for Seb1-mCherry (a), sme2 RNA-U1Atag/U1Ap-mCherry (b), and A55-lacO/LacI-GFP (c). The sme2 RNA-U1Atag was visualized by U1Ap-mCherry¹¹. Three-dimensional images were captured every minute. Microfluidic yeast plates (CellASIC) were used for cell culture on the microscope stage. 1,6-Hexanediol (10%) was added or removed, as indicated by the arrows. The solution in the cell culture chamber was changed at <1 min. Scale bar, 5 μm. d Number (%) of cells with paired chromosomes at sme 2 (left), A55 (middle), and C24 (right) loci. 1,6-Hexanediol was added to WT and rec12 deleted (rec12⁻) cells at 5 min and removed at 8 min (treatment period is shaded in the graphs). Three-dimensional live cell images were captured every minute, and the percentage of paired cells observed at 5-min intervals is shown. Source data for Fig. 5 are provided as a Source Data file 5.