Skip to main content
. Author manuscript; available in PMC: 2019 Dec 7.
Published in final edited form as: Mol Cell. 2019 Sep 18;76(5):699–711.e6. doi: 10.1016/j.molcel.2019.08.017

Figure 1. Role of MRN, Ctp1, Exo1, and Rqh1 in Initial and Extensive Resection.

Figure 1.

(A and B) Southern blot analysis of initial resection (0–150 min) (A) and extensive resection (0–12 h) (B) at lys1 locus in wild-type, mre11, and ctp1 cells.(C and D) Southern blot analysis of initial resection (0–150 min) (C) and extensive resection (0–12 h) (D) at lys1 locus in wild-type and exo1, rqh1, or exo1 rqh1 mutant cells. Restriction-enzyme-digested DNA was separated on 0.8% agarose gels. Primers used to prepare DNA probes for Southern blotting and restriction enzymes used to digest genomic DNA are presented in Table S3. Plots show kinetics of resection and error bars denote SD (n = 3). An asterisk in (C) denotes unresected DSB end.