Skip to main content
. Author manuscript; available in PMC: 2019 Dec 7.
Published in final edited form as: Mol Cell. 2019 Sep 18;76(5):699–711.e6. doi: 10.1016/j.molcel.2019.08.017

Figure 4. Analysis of DNA-Binding Domain of Rad52 in Resection Control.

Figure 4.

(A) Sequence comparison of yeasts and human Rad52.

(B) Schematic of new SSA assay between two ~0.75-kb λ2 repeats inserted at arg1 locus and 21 kb upstream of arg1.

(C) Southern blot analysis of SSA in indicated rad52 mutants deficient in DNA binding (rad52-R45A) or C-terminal domain (rad52ΔC80). Plot shows kinetics of SSA product formation. Error bars denote SD (n = 3).

(D) Comparison of extensive resection kinetics in wild-type and rad52-R45A mutant cells. Plots show kinetics of resection, and error bars denote SD (n = 3).