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. Author manuscript; available in PMC: 2019 Dec 7.
Published in final edited form as: Mol Cell. 2019 Sep 18;76(5):699–711.e6. doi: 10.1016/j.molcel.2019.08.017

Figure 5. Rad52 Controls Rqh1-Mediated Resection and Rqh1 Recruitment.

Figure 5.

(A) Epistasis analysis between Rad52 and enzymes mediating two extensive resection pathways, Exo1 and Rqh1. Southern blot analysis of resection in indicated mutants. Plots show kinetics of resection, and error bars denote SD (n = 3).

(B and C) ChIP-qPCR analysis of Rqh1 and Exo1 loading in wild-type and rad52 cells at a DSB at lys1 locus at 1 and 10 kb from DSB ends. Error bars denote SD (Rqh1-FLAG: wild type [WT], n = 7, rad52Δ, n = 8. Exo1-FLAG: n = 3). (B) One-tailed p values were shown (**p < 0.01, ***p < 0.001). (C) One-tailed p values were shown (*p < 0.05).