KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Actin | Cell signaling | 4970S |
| Cyclophilin B | Proteintech | 11607-1-AP |
| LKB1 | Cell signaling | 3047S |
| E-cadherin | Cell signaling | 3195P |
| Vimentin | Cell Signaling | 5741P |
| p-EGFR (Y1068) | Cell signaling | 3777P |
| Brdu | ThermoFisher | BDB347583 |
| IDH1 | Shanghai Genomics, Inc. | N/A |
| p-IDH1 (Y42) | Shanghai Genomics, Inc. | N/A |
| p-IDH1 (Y391) | R & D Systems | Cat# MAB7049 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| pemetrexed (cell line validation) | Medchem Express | Cat# HY-10820A |
| pemetrexed (xenograft validation) | Eli Lilly | N/A |
| [U-13C] glucose | Cambridge Isotopes | Cat# CLM-1396 |
| [3,4-13C] glucose | Cambridge Isotopes | CLM-6750 |
| [U-13C] glutamine | Cambridge Isotopes | CLM-1822 |
| Puromycin | Sigma | Cat# P8833 |
| Critical Commercial Assays | ||
| Pierce™ BCA Protein Assay Kit | ThermoFisher | Cat# 23225 |
| Deposited Data | ||
| NSCLC cell line microarray data | (Kim et al., 2016) | GEO: GSE32036 |
| NSCLC cell line mutation data | (McMillan et al., 2018) | described in table S4 |
| NSCLC cell line RPPA data | This paper | described in table S4 |
| NSCLC cell line methylation data | (Walter et al., 2012) | described in table S4 |
| NSCLC cell line Erlotinib sensitivity data | (McMillan et al., 2018) | N/A |
| NSCLC cell line Pemetrexed sensitivity data | This paper | described in table S4 |
| Experimental Models: Cell Lines | ||
| NSCLC cell line collection | This paper | described in table S4 |
| Experimental Models: Organisms/Strains | ||
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJArc | UT Southwestern | RRID:IMSR_ARC:NSG |
| Recombinant DNA | ||
| pLKO.1 | Open Biosystems | N/A |
| pCMVΔR8.91 | (Cheng et al., 2011) | N/A |
| pMD2.G | (Cheng et al., 2011) | N/A |
| MigCD8t | (Faubert et al., 2014) | N/A |
| MigCD8t-LKB1 | (Faubert et al., 2014) | N/A |
| Oligonucleotides | ||
| shPC (CCGGGCCAAGGAGAACAACGTAGATCTCGAGATCTACGTTGTTCTCCTTGGCTTTTTG) | (Cheng et al., 2011) | N/A |
| Software and Algorithms | ||
| GC MSD Chemstation | Agilent | https://www.agilent.com/en/products/software-informatics/massspec-workstations/gc-msd-chemstation-software |
| R Version 3.3.2 | R Core Team, 2016 | https://www.r-project.org/ |
| Gene Set Enrichment Analysis | (Subramanian et al., 2005) | http://software.broadinstitute.org/gsea/downloads.jsp |
| GSVA | (Hanzelmann et al., 2013) | https://bioconductor.org/packages/release/bioc/html/GSVA.html |
| drc | (Ritz et al., 2015) | https://cran.r-project.org/web/packages/drc/index.html |
| Other | ||
| Fetal bovine serum | Gemini Bio-Products | Cat# 100-106 |
| Dialyzed fetal bovine serum | Gemini Bio-Products | Cat# 100-108 |
| Tri-Sil HTP reagent | ThermoFisher | N/A |
| Difco™ Noble Agar | VWR | N/A |
| ECL Western Blotting Substrate | ThermoFisher | Cat# 32106 |
| PolyJet™ In Vitro DNA Transfection Reagent | SignaGen Laboratories | N/A |