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. Author manuscript; available in PMC: 2020 Jun 2.
Published in final edited form as: Nat Med. 2019 Dec 2;25(12):1938–1947. doi: 10.1038/s41591-019-0668-z

Extended Data Fig. 3. ABT263 or VHL-L blocks the formation of ternary complex of BCL-XL, DT2216 and VHL.

Extended Data Fig. 3

a, Recombinant His-tagged BCL-XL protein (100 nM) was incubated with the VHL-Elongin B/C complex (50 nM) with 0.039 μM of DT2216. The ternary complex formation was abrogated in the presence of ABT263 (1 μM) or VHL-L (10 μM). Data are expressed as mean (n = 2 technical replicates). Each symbol represents data from an individual replicate. b, The negative-control of DT2216 (DT2216NC) cannot form ternary complex with BCL-XL and the VHL-complex. Recombinant His-tagged BCL-XL protein (100 nM) was incubated with the VHL-Elongin B/C complex (50 nM) with increasing concentrations of DT2216 or DT2216NC. Data are expressed as mean (n = 2 technical replicates).