Fig. 4.
A combined innate and adaptive immunotherapeutic approach leads to 9464D-GD2 tumor regression and immunological memory. a Tumor growth curves are shown for TAC mice bearing intradermal 9464D-GD2 tumors (about 50mm3) that were untreated or treated with RT alone or RT and combined ½ dose IT-IC, anti-CTLA-4 (CTLA), CpG and anti-CD40 (CD40). Tumor-free mice on day 60 are denoted as number of CR of total mice in the group. b) Photographs of 3 representative TAC mice per group taken on day 24 show contrasting tumor size and appearance after 12 Gy alone or 12 Gy and immunotherapy (ITx, or combined ½ dose IT-IC, anti-CTLA-4, CpG, and anti-CD40) compared to untreated control mice. c Mice previously bearing a 9464D-GD2 tumor on the right flank that had complete response to treatment were rechallenged on day 90 by injecting 9464D-GD2 cells into the left flank. Tumor volumes on day 30 after tumor cell injection are significantly larger for naïve mice compared to previously treated mice (p = 0.0003)