Fig. 7.

iRGD-PS-Tam do not accumulate in normal mammary epithelial tissue adjacent to the tumor site. a Mice bearing s.c. M05 tumors were i.v. injected with iRGD-FAM-PS-Tam. After 4 h the animals were sacrificed and tumors and adjacent mammary gland were collected and snap-frozen. Immunofluorescence staining with anti-NRP-1 and FAM primary antibodies was performed. Nuclei were counterstained with RedDot. Representative images of tumors and normal mammary gland from two independent experiments are shown, where nuclei are shown in red and specific staining in green. Scale bars: 20 μm. b T47D and HC11 cells were seeded on glass coverslips in 24-well plates. After 24 h, iRGD-FAM-PS-Tam were added to the cells at a concentration of 0.5 mg/mL and incubated for 1 h. PS are labelled in green and nuclei are counterstained with RedDot (red). Scale bars: 50 μm. Quantification of the fluorescence intensity/cell for T47D and HC11 cells. No statistically significant difference was detected between both cell lines; graph shows PS fluorescence in arbitrary units; bars represent mean ± SEM; N = 3; student’s t test was performed to analyze statistical significance