Fig. 1.

Promoter DNA hypermethylation mediates the downregulation of miR-484 expression in CC. a The mRNA level of miR-484 in CC cell lines after treatment with 5-Aza-CdR was measured by RT-qPCR. b The diagram shows the promoter region of the miR-484 gene and the CpG island located within this region. The red vertical bar represents the CpG sites. c and d Luciferase reporter system was used to detect the promoter activity of miR-484 in CC cell lines (c) and after 5-Aza-CdR treatment (d). e genomic bisulfite sequencing was performed to determine the methylation status of the miR-484 promoter in 10 pairs of CC tissues (T1-T10). f genomic bisulfite sequencing was performed to determine the methylation status of the miR-484 promoter in CC cell lines after 5-Aza-CdR treatment. The black circle indicates methylated CpG loci and the white circle indicates unmethylated CpG loci. g Scatter plots showing miR-484 expression compared with methylation. Error bars in a, c, and d indicate the mean ± SD of three independent experiments. **p < 0.01