Fig. 3.

miR-484 is repressed by hypermethylation mediated by EZH2-recruited DNMT1. a Schematic location of the core DNA motif (red) of the Polycomb response element (PRE). b, c PCR (b) and CHIP-qPCR (c) assay for anti-EZH2 and H3K27me3 in CC cells. d Western blot analysis of EZH2, H3K27me3, and Histone3 in CC cells with EZH2 overexpression or downregulation or control. e, f CHIP-qPCR assays for CC cells after EZH2 modification. g Physical interactions between EZH2 and DNMT1 were examined via a Co-IP assay in CC cells with EZH2 overexpression or downregulation. h CHIP-qPCR assays for anti-DNMT1 in CC cells after EZH2 downregulation. Mean (n = 3) ± SD. Student’s t test, *p < 0.05, **p < 0.01