Fig. 8.

miR-484 negatively regulates the WNT/MAPK and TNF signaling pathway in CC cells. a and d Western blot were used to measure the protein levels after transfection with pri-miR-484 or ASO-miR-484. b Western blot showed the protein levels in cells treated with pFlag-HNF1A (HNF1A) or shR-HNF1A. c HeLa and C33A cells were co-transfected with pTOP/flash or pFOP/flash, pcDNA3, pri-miR-484, pFlag-HNF1A, or pri-miR-484 and pFlag-HNF1A, respectively, after which luciferase reporter assays were performed. e Western blot showed the protein levels in cells treated with pFlag-MMP14 (MMP14) or shR-MMP14. All of the experiments were repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001. f The model by which DNMT1 epigenetically mediates miR-484 repression with resultant MMP14/HNF1A activation, leading to tumor malignancy in CC