Fig. 4.

DDX56-mediated virus-triggered signaling at the level of IRF3. (A,B) Effects of DDX56 on the IFN-β promoter and ISRE activation by various signaling components. 293T cells (10⁵) were transfected with the IFN-β promoter (A) or ISRE (B) reporter (0.1 µg), and the expression plasmids for DDX56 and the indicated plasmids (0.1 µg each). Luciferase assays were performed 24 h after transfection. Shown are representative experiments of three independent experiments with mean±s.d. of three technical replicates. *P<0.05, **P<0.01. EV, empty vector; Luc, luciferase; Con, control.