Fig. 6.

DDX56 inhibits the nuclear translocation of IRF3. (A) Overexpression of DDX56 inhibits nuclear translocation of IRF3. Stably transfected DDX56 293T cells (5×10⁷) were left untreated or treated with SeV for the indicated time. Cell fractionation was performed, and the cytoplasmic and nuclear proteins were prepared in 1 ml of homogenization buffer or nuclear extraction buffer, respectively, followed by co-immunoprecipitation and immunoblotting (WB) analysis. (B) Effects of DDX56 knockdown on IRF3 nuclear translocation. The DDX56-RNAi stable cells (5×10⁷) were left untreated or treated with SeV for the indicated time points. Experiments were performed similar to those in A. (C) DDX56 deficiency increases nuclear translocation of IRF3 in HeLa cells. Experiments were performed in a similar manner to those in A. (D) Effects of DDX56D166N and DDX56E167Q on the nuclear translocation of IRF3. Experiments were performed in a similar manner to those in A. EV, empty vector; WT, wild type; Coni, control siRNA.