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. 2019 Nov 18;146(22):dev180034. doi: 10.1242/dev.180034

Fig. 6.

Fig. 6.

RNT-1/BRO-1 can overrule Wnt signaling by lowering pop-1 expression levels. (A) Gene map of the tagged endogenous gfp::pop-1 allele. (B) Time-lapse spinning disk confocal microscopy images of GFP::POP-1 and seam cell markers mCherry::PH and Cherry::H2B during L2 symmetric, L2 asymmetric and L3 asymmetric divisions in the wild type, and L2 symmetric division in rnt-1(tm388) bro-1(tm1183) mutants. Arrowheads point to daughter cell nuclei. Time is indicated in minutes relative to metaphase. (C) Quantification of the A:P ratio of GFP::POP-1 in daughter cell nuclei of wild-type L2 and L3 divisions, and L2 symmetric division in rnt-1(tm388) bro-1(tm1183) mutants. n≥15 cells. (D) Relative expression levels of GFP::POP-1 during L2 and L3 divisions in the wild type, and L2 symmetric division in rnt-1(tm388) bro-1(tm1183) mutants. n≥15 cells. (E) Spinning disk confocal microscopy images of normal control and heat shock-induced RNT-1/BRO-1 late L2 animals. Seam markers are mCherry::PH and Cherry::H2B. (F) Relative GFP::POP-1 expression levels in control animals and heat shock-induced RNT-1/BRO-1 animals. n≥40 animals. (G) GFP::POP-1 nuclear A:P ratio in control animals and heat shock-induced RNT-1/BRO-1 animals. n≥40 animals. Note that the images in D and F were taken with different settings, therefore the relative levels (y-axis) are different between these experiments. Data are mean±s.d. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001; unequal variance (Welch's) t-test. Scale bars: 10 µm (B); 20 µm (E).