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. 2019 Nov 4;8(11):bio045906. doi: 10.1242/bio.045906

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — Tube formation assays in normal and PPHN PAEC. Representative high-throughput imaging assays of nuclei (purple), actin (blue), eNOS (green) and VEGF (red) in 18 h tube-formation assays for (A) normal PAEC in vehicle media, (B) normal PAEC in IGF-1 media, (C) PPHN PAEC in vehicle media and (D) PPHN PAEC in IGF-1 media. Two replicates performed from different cell isolations for each normal and PPHN PAEC are presented here.