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. 2019 Nov 12;8(11):bio046789. doi: 10.1242/bio.046789

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Confocal micrographs showing phenotypes of selected genes after being knocked down using the UAS-Dcr2, w¹¹¹⁸; nosP-GAL4-NGT40; bamP-GFP driver. (A) Control flies are driver flies crossed with wild type (w⁻). The anterior part of a single ovariole with maturing egg-chambers stained for Vasa, GFP (Bam) and alpha-spectrin is shown. (B-E) RNAi knockdown phenotypes for cav (B,B′), CG4038 (C,C′), Tusp (D,D′) and myc (E) stained as in A. Panels labeled with ' are higher magnification images of the respective genotypes. B′ is a digital magnification of B at a slightly different focal plane to illustrate non-specific anti-Vas staining within and around the apparently empty germaria. (B–E) The anterior tips of whole ovaries containing several ovarioles are shown. Anterior is to the left. Scale bars: 10 µm.