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. 2019 Nov 26;146(22):dev179333. doi: 10.1242/dev.179333

Fig. 4.

Fig. 4.

E2FA and E2FB are dispensable for embryonic cell proliferation. (A) Representative confocal images of mature embryos from wild type (WT) and e2fa-2, e2fb-1 single and e2fa-2/e2fb-1 double mutants dissected from mature dry seeds. (B) Confocal images of propidium iodide (PI)-stained WT-Col and e2fa-2/e2fb-1 double mutant embryos (additional images are shown in Fig. S5). White boxes outline epidermal regions in cotyledons and hypocotyls of WT and e2fa-2/e2fb-1 double mutants, enlarged on the right to show epidermal cell sizes. Scale bars: 100 µm. (C,D) The entire cotyledon (C) and hypocotyl (D) area of mature dried embryos was measured. Data are mean±s.d., n=3 biological repeats, N=10 samples in each. Cell size and cell number were calculated using ImageJ. Sample size N≥200 cells/image (n=4 biological repeats). *P≤0.05, **P≤0.01, ***P≤0.001 (two-tailed, paired t-test between the corresponding mutant and the WT). ns, non-significant.