Figure 1. Latent membrane protein 1 (LMP1) promotes interleukin-8 (IL8) production in a human gingival epithelial cell line. Ca9-22 cells were transfected with pSG-LMP1 (0.2 μg) or pSG (0.2 μg) as control vector (ContV) for different times (A, C) and with pSG-LMP1 at different concentrations (0.05, 0.1, or 0.2 μg) for 24 h (B, D). A, B: The cells were harvested and the level of IL8 mRNA was determined using reverse transcription polymerase chain reaction analysis with specific primers. C, D: IL8 released into the culture supernatants was determined using enzyme-linked immunosorbent assay. The values are presented as mean±SD; n=3. **Significantly different at p<0.0001).