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. Author manuscript; available in PMC: 2020 Jun 2.
Published in final edited form as: Nat Struct Mol Biol. 2019 Dec 2;26(12):1167–1175. doi: 10.1038/s41594-019-0344-5

Extended Data Fig. 1. Mass spectrometry characterization of E51 Fab.

Extended Data Fig. 1

a, Peak fractions from anion exchange chromatography of E51 Fab. b, Mass spectra of the three E51 peaks from panel a. The molecular mass for Peak 1 corresponded within 27 Da to the predicted mass for unmodified E51 Fab (48,791 Da), suggesting this E51 Fab fraction contained no sulfated tyrosines. The molecular masses for Peaks 2 and 3 were each increased by 80 Da from the preceding peak, corresponding to the molecular weight of a SO3− group (80 Da). These results are consistent with the identification of Peaks 2 and 3 as E51 Fab with one and two sulfated tyrosines, respectively. Peak 3 was used for preparing complexes for cryo-EM.