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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Cell Physiol. 2019 Sep 19;235(3):2350–2365. doi: 10.1002/jcp.29141

Figure 9. IL-10 prevents high glucose-mediated suppression of osterix expression via the ERK pathway.

Figure 9.

(A) MC3T3-E1 cells were treated with high glucose (72 hours) +/− IL-10 for 24 hours. RNA extracted and mRNA analysis of osterix (OSX) gene assessed by Q-RT-PCR and normalized to HPRT. (B) MC3T3-E1 cells were treated with high glucose (72 hours) +/− IL-10 for 15 minutes. Representative blots show protein levels analyzed by Western Blot as well as loading controls (ERK and Tubulin). Quantification of western blots for p-ERK/ERK, p-p38, and p-JNK. Values are averages ± standard error; n= 4–5 per group, *p<0.05, **p<0.01. Statistical analysis performed by One-way ANOVA followed by Fisher’s LSD post-test.

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