Figure 1.

Percentage of TLR2 (a) and TLR4 (b) expression on the surface of CD16+ human polymorphonuclear neutrophils (PMNs). PMNs were incubated in the absence (control) or presence of gp43 (20 ng/ml) for 4 h, and PMNs were analyzed using flow cytometry. Results are presented as percentage of PMNs expressing TLR2 and TLR4 using box-and-whiskers (min to max) graphs with data from 7 women tested, considering p < 0.05. Statistically significant differences between groups are indicated as follows: ^∗p < 0.05 and ^∗∗p < 0.01. (c) Representative experiment showing the expression of PMN (control culture) CD16 PerCP+, TLR2 FITC+, and TLR4 PE+, and histograms display the percentage of TLR2 and TLR4 blockade (red line) and the remaining positive receptor expression (gray lines).