Skip to main content
. 2019 Nov 25;2019:2345658. doi: 10.1155/2019/2345658

Figure 2.

Figure 2

PRMT5 inhibition attenuated renal injury and promoted tubular cell proliferation after I/R. SCr levels (a) and BUN levels (b) were detected after intraperitoneal injection of EPZ with three concentrations (5 mg/kg, 10 mg/kg, and 20 mg/kg) daily for 7 days in normal mice. SCr levels (c) and BUN levels (d) were detected after intraperitoneal injection of EPZ with three concentrations (5 mg/kg, 10 mg/kg, and 20 mg/kg) daily for 7 days before mice undergoing I/R. Scores for the histological appearance of acute tubular necrosis (H) and representative images of mouse kidney H-E staining (original magnification ×400) (e). The representative of immunoblotting of PRMT5 (f) and Ki-67 (g) (original magnification ×400). IOD/area indicated the expression of PRMT5 (i) and the number of Ki-67-positive cells/field indicated the tubular cell proliferation (j). (k) PRMT5 protein levels were detected by western blot analysis. Values were expressed as the mean ± SEM. ∗P < 0.05, relative to the sham group; #P < 0.05, relative to the I/R group; ΔP < 0.05, relative to the 5 mg/kg group; &P < 0.05, relative to the 10 mg/kg group, n = 6. BUN: blood urea nitrogen; SCr: serum creatinine; H-E: hematoxylin-eosin; I/R: ischemia-reperfusion; EPZ: EPZ015666, PRMT5 inhibitor.