Figure 3.

PRMT5 inhibition downregulated oxidative stress and pyroptosisi-related proteins induced by renal I/R. (a) PRMT5 protein levels and (b) pyroptosis-related protein levels were detected by western blot analysis after administration of EPZ. The representative of immunoblotting of caspase-1 (c) (original magnification ×400). IOD/area indicated the expression of caspase-1 (d). (e) SOD activation. (f) MDA contents. Values were expressed as the mean ± SEM. ^∗P < 0.05, relative to the sham group; ^#P < 0.05, relative to the I/R group. n = 6. I/R: ischemia-reperfusion; EPZ: EPZ015666, PRMT5 inhibitor; SOD: superoxide dismutase; MDA: malondialdehyde.