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. 2019 Nov 25;2019:2345658. doi: 10.1155/2019/2345658

Figure 4.

Figure 4

Pyroptosis induce by H/R in HK-2 cells depends on oxidative stress. (a, c) The levels of oxidative stress in different reoxygenation time groups of HK-2 cells was measured by flow cytometry. (b) The cell viability in different reoxygenation time groups of HK-2 cells was measured by CCK-8. (d) An Amplex Red assay showed that hydrogen peroxide was increased with the reoxygenation time extension. (e) Pyroptosis-related protein levels were detected by western blot analysis after different reoxygenation time. (f–i) The apoptosis and the levels of oxidative stress in different intervention groups of HK-2 cells were measured by flow cytometry. (j) Pyroptosisi-related protein levels were detected by western blot analysis after different interventions. Values were expressed as the mean ± SEM. (b–e) ∗P < 0.05, relative to the control group; #P < 0.05, relative to the reoxygenation time of the 2 h group; ΔP < 0.05, relative to the reoxygenation time of the 3 h group, n = 6. (h–j) ∗P < 0.05, relative to the control group; #P < 0.05, relative to the H/R group, n = 6. H/R: hypoxia/reoxygenation; ROS: reactive oxygen species; NAC: N-acetyl-L-cysteine.