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. 2019 Nov 25;2019:1305049. doi: 10.1155/2019/1305049

Figure 2.

Figure 2

AAA-ASCs displayed increased cellular senescence. (a) Cell growth curves show the lower proliferative ability of AAA-ASCs compared to that of H-ASCs. (b) Representative images of cell morphology and quantitative analysis of cell size in H-ASCs and AAA-ASCs. Scale bar = 200 μm. (c) Representative images of SA-β-gal staining and quantitative analysis of SA-β-gal-positive cells in H-ASCs and AAA-ASCs. Scale bar = 200 μm. (d) Western blotting and quantitative analysis of the expression levels of p53 and p21 in H-ASCs and AAA-ASCs. (e) Immunostaining of the proliferation marker Ki67 and quantitative analysis of Ki67-positive cells in H-ASCs and AAA-ASCs. Scale bar = 200 μm. Data are expressed as mean ± SEM. n = 3. ∗∗∗p < 0.001.