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. 2019 Nov 25;2019:1305049. doi: 10.1155/2019/1305049

Figure 4.

Figure 4

Mitochondrial function was decreased in AAA-ASCs. (a) Representative images of mitochondrial morphology determined by MitoTracker staining in H-ASCs and AAA-ASCs. Scale bar = 25 μm. (b) Western blotting and quantitative analysis of the expression level of p-Drp1 ser616/Drp1 and Mfn2 in H-ASCs and AAA-ASCs. (c) Representative images of ROS determined by MitoSOX staining and quantitative analysis of ROS generation in H-ASCs and AAA-ASCs. Scale bar = 100 μm. (d) Representative images of MMP determined by JC-1 staining and quantitative analysis of MMP in H-ASCs and AAA-ASCs. (e) The intracellular ATP level in H-ASCs and AAA-ASCs. Data are expressed as mean ± SEM. n = 3. ∗∗∗p < 0.001.