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Effects of L-Cth on apoptosis, caspase-3 activities, and mitochondrial superoxide generation in Hcy-treated HUVECs. (a) HUVEC apoptosis detected by TdT-mediated dUTP nick end labeling (TUNEL) methods (magnification, ×600; scale bar: 20 μm). (b) Cleavage of caspase-3 analyzed by western blotting. (c) Mitochondrial superoxide generation in HUVECs detected with MitoSOX. Data are presented as mean ± SEM. ∗∗P < 0.01 versus control group; ##P < 0.01 versus Hcy group. L-Cth: L-cystathionine.
